تاثیر مورفین خوراکی بر بافت کبد جنین موش صحرائی نژاد ویستار در هفته دوم جنینی: یک مطالعه هیستوپاتولوژیک

 Background: More than 90% of women use drugs during pregnancy. The aim of this study was to investigate oral morphine-induced histopathological changes in Wistar rat fetuses’ liver. Methods: Animals were divided to 1 control and 3 drug level induced groups. 0.1, 0.2 and 0.3 mg/ml of morphine sulphate (99.98%) in drinking water (25 ml) were administered orally to pregnant rats from day 8 to 20. On gestation day 21, fetuses were removed surgically and after removing liver of fetuses, samples of tissue stained by hematoxylin and eosin (H&E) method and studied under light microscope. The data evaluated by SPSS software, ANOVA and analysis varieties. Results: In the experimental groups with dose 0.1, 0.2 and 0.3 mg/ml of morphine, liver sections appeared with vacuolated hepatocytes, dilated sinusoids, and increased number of kupffer cells, lymphocytes and neutrophils. The results in experimental groups with doses 0.2 and 0.3mg/ml of morphine were significant comparing with control group (p≤ 0.001). Conclusion: The results indicate that the lowest dose of morphine (0.1mg/ml) induced very low toxicity in fetuses but the middle dose (0.2mg/ml) and the high dose of morphine (0.3mg/ml) induced adverse reactions. It is concluded that oral morphine sulphate induces significant histopathological changes in the liver tissue, and the severity of these changes increases with time, period and dosage